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In vitro transcription with purified recombinant HrcA. (A) Transcription of single promoters. The upper transcript is from the dnaK promoter; the lower transcript is from the omcB promoter. Lanes 1 and 3 (counting from the left), no HrcA; lanes 2 and 4, 1,150 nM HrcA present. (B) Cotranscription of the dnaK and omcB promoters. Lane 1, no HrcA; lane 2, 230 nM HrcA; lane 3, 460 nM HrcA; lane 4; 1,150 nM HrcA.
