TABLE 1.
Strains used in this work
| Strains | Characteristics or use | Origin or reference |
|---|---|---|
| S. clavuligerus | ||
| ATCC 27064 | Wild type | ATCCa |
| ccaR::aph | Cephamycin C, clavulanic acid-nonproducer (CCA−), disruption in ccaR, kanamycin resistant (Kmr) | 25 |
| claR::aph | Clavulanic acid nonproducer (CA−), disruption in claR | 26 |
| ORF6::apr | Disruption in ORF6, apramycin resistant (Aprr) | 7 |
| ORF6::apr ccaR::aph | CCA−, disruption in ccaR and ORF6, Kmr Aprr | 7 |
| ceaS::aph | CA−, disruption in ceaS, Kmr | 27 |
| RLF3 | CA−, disruption in bls, Kmr | 2 |
| car::aph | CA−, disruption in car, Kmr | 26 |
| cyp::aph | CA−, disruption in cyp, Kmr | 17 |
| ORF12::aph | CA−, disruption in ORF12, Kmr | 17 |
| ORF15::aph | CA−, disruption in ORF15, Kmr | 17 |
| ORF15::aph (pIJ699) | CA−, disruption in ORF15, Kmr Thior | 16 |
| ORF15::aph (pIJ699-ORF15) | CA−, disruption in ORF15, complemented, Kmr Thior | 16 |
| cyp::aph (pVK99) | CA−, disruption in cyp, Kmr Thior | 16 |
| cyp::aph (pVK99-cyp) | Disruption in cyp, complemented, Kmr Thior | 16 |
| Micrococcus luteus ATCC 9341 | Sensitive strain in bioassay | ATCC |
| Bacillus sp. strain 27860 | Sensitive strain in bioassay | ATCC |
| Escherichia coli Ess22-31 | Sensitive strain in bioassay | 26 |
| Klebsiella pneumoniae 29665 | Sensitive strain in bioassay | ATCC |
| Alcaligenes faecalis | Sensitive strain in bioassay | Laboratory stock |
| Enterobacter cloacae P99 | Wide-spectrum cephalosporinase producer | Laboratory stock |
a
ATCC, American Type Culture Collection.