FIG. 2.

RT-PCR analysis of the dofA and dofB mRNAs in M. xanthus. Total RNAs prepared from M. xanthus vegetative cells (V, lanes 2, 6, 11, and 15) and developing cells at 6 h (lanes 3, 7, 12, and 16), 12 h (lanes 4, 8, 13, and 17), and 24 h (lanes 5, 9, 14, and 18) poststarvation were treated with RT (+RT, lanes 2 to 5 and 11 to 14) or not treated with RT (−RT, lanes 6 to 9 and 15 to 18) and subjected to PCR by using the appropriate primers for cDNAs of dofA (lanes 1 to 9) and dofB (lanes 10 to 18). The PCR products were analyzed by 6% PAGE followed by ethidium bromide staining. Lanes C (lanes 1 and 10), PCR product amplified from the M. xanthus chromosome. Lane M, molecular weight markers. The molecular sizes of DNA fragments are indicated at the left in base pairs.