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. 2002 Dec;184(24):6803–6810. doi: 10.1128/JB.184.24.6803-6810.2002

FIG. 4.

FIG. 4.

Transcription initiation sites of dofA and dofB mRNAs. Total RNAs (100 μg) prepared from M. xanthus vegetative cells (V) and developing cells at 24 h poststarvation (D) were subjected to primer extension analysis. The products of primer extension with appropriate primers are marked by arrows to the right of each panel. (A) dofA mRNA. (B) dofB mRNA. Lanes G, A, T, and C, sequence ladders generated by the dideoxy chain termination method with the same primers and with pSP11 or pGEM-dofB DNA as a template. The DNA sequences around the transcription initiation sites (bent arrows) are indicated to the left of each panel. The −10 sequences of the putative promoters are boxed.