TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
| Strains | ||
| E. coli DH5α | supEA4 ΔlacU169 (φ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-I relA1 | 19 |
| M. xanthus | ||
| DZF1 | sglA1 | 23 |
| MO1 | fruA::Tc Ω5 | 32 |
| DK731 | csgA731 | 37 |
| DK3068 | dsgA439 TcrsglA1 | 11 |
| JD275 | esg::Tn5 Ω258 Kmr | 11 |
| JD276 | asgA476 Kmr | 11 |
| JD278 | bsgA330 Tcr | 11 |
| TH1 | sglA1 ΔdofA::Km | This work |
| TH2 | sglA1 ΔdofB::Km | This work |
| TH3 | sglA1 ΔdofA::Km ΔdofB::Tc | This work |
| MxDAL | sglA1 dofA::pDAL-Km | This work |
| MxDAL-A | asgA476 KmrdofA::pDAL-Tc | This work |
| MxDAL-B | bsgA330 TcrdofA::pDAL-Km | This work |
| MxDAL-C | csgA731 dofA::pDAL-Km | This work |
| MxDAL-D | dsgA439 TcrsglA1 dofA::pDAL-Km | This work |
| MxDAL-E | esg::Tn5 Ω258 KmrdofA::pDAL-Tc | This work |
| MxDAL-F | fruA::Tc Ω5 dofA::pDAL-Km | This work |
| MxDAL-2 | sglA1 dofA::pDAL-2 | This work |
| MxDAL-2F | sglA1 fruA::Tc Ω5 dofA::pDAL-2 | This work |
| MxDAL-2C | sglA1 csgA731 dofA::pDAL-2 | This work |
| MxDBL | sglA1 dofB::pDBL | This work |
| Plasmids | ||
| pGEM-T easy | AprlacZ cloning vector | Promega |
| pMC1403 | AprlacZ translational fusion vector | 5 |
| pMC1403Km | KmrlacZ translational fusion vector; derivative of pMC1403 | This work |
| pMC1403Tc | TcrlacZ translational fusion vector; derivative of pMC1403 | This work |
| pSI1403 | KmrlacZ transcriptional fusion vector; derivative of pMC1403 | 20 |
| pSI1403attP | Kmrint attP lacZ transcriptional fusion vector; derivative of pSI1403 | 20 |
| pSP11 | 1.1-kb HincII fragment containing dofA of DZF1 in pUC19 | 20 |
| pGEM-dofB | 3,032-bp dofB fragmenta in pGEM-T easy | This work |
| pDAL-Km | 1,135-bp dofA upstream fragmenta in pMC1403Km | This work |
| pDAL-Tc | 1,135-bp dofA upstream fragmenta in pMC1403Tc | This work |
| pDAL-2 | 1,135-bp dofA upstream fragmenta in pSI1403 | This work |
| pDBL | 1,255-bp dofB upstream fragmenta in pMC1403Km | This work |
a
DNA fragments were synthesized by PCR with M. xanthus DZF1 chromosomal DNA.