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PurifiedβH25-31fragments form homodimers.A, purified βH25-31 runs as a dimer on a nondenaturing gel. B, the top-ranked predicted AlphaFold2 model for βH25-31 is a dimer with anti-parallel chains (colored from N-terminus in blue to C-terminus in red). C, a surface view of the AlphaFold2 structure colored by electrostatic potential. The two chains have been pulled apart and rotated to show the binding interface to show a pattern of complementary charges.
