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. 2002 Dec;184(24):7025–7041. doi: 10.1128/JB.184.24.7025-7041.2002

FIG.2.

FIG.2.

L. pneumophila morphology in the prereplicative phase. (A) Plate-grown legionellae (strain 2064 shown) with a standard gram-negative morphology, used as the inoculum to infect HeLa cells. (B) The overall bacterial morphology did not change during binding or internalization (strain SVir at 6 h postinfection shown). (C) More than one morphologically unchanged bacterium (SVir shown) could colocalize in early vacuoles. (D) Morphologically unchanged bacterium (SVir shown) in a vacuole surrounded by mitochondria (m) and vesicles. (E) HeLa-grown legionellae (SVir shown) tightly bound to the surface of a HeLa cell. Note the unique envelope morphology (multimembranous envelope) (arrows) of the bound bacteria and the prominent inclusion (Inc) in one of them. (F) HeLa-grown legionellae (2064 shown) inside an early vacuole surrounded by a multitude of vesicles. Note the bubble in the bacterial cytoplasmic region produced by a poorly embedded inclusion. Bars, 0.5 μm.