FIG. 7.

In vitro intermediates of the MIF-to-replicative-form transition. (A) Strain 2064 intermediate depicting intraperiplasmic vesicles (arrow). (B) An SVir intermediate with several intraperiplasmic vesicles (arrow) is shown side by side with a section of a replicating bacterium depicting a standard gram-negative ultrastructure. (C) Typical replicative 2064 forms derived from MIFs ≈20 h after inoculation into BYE broth. (D) Stationary-phase 2064 intermediate with multiple internal membranes but a generally unresolved envelope. Bars, 0.5 μm.