FIG. 9.

Freeze fracture analysis of agar-grown and postreplicative SVir forms. (A and B) Fracture replicas of MIFs, showing both cytoplasmic inclusions and an uneven distribution of intramembranous particles in the inner membrane. (C) Fracture replicas of agar-grown bacteria. Notice the lack of inclusions in the exposed fractures across the cytoplasm and the even distribution of intramembranous particles in the inner membrane. (D) Fracture replicas of MIFs after they were grown on BCYE plates to form a visible film. It was common to see small vesicles in areas corresponding to the periplasm (arrowheads) and inner membranes with evenly distributed intramembranous particles. Bars, 1.0 μm.