TABLE 3.
Plasmids used in this study
| Plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| pKS | Cloning vector | Stratagene |
| pUC18 | Cloning vector | 47 |
| pTrcHisB | Vector for production of His-tagged fusion | Invitrogen |
| pUC4DEcat | cat gene used in H. influenzae cloning Chlorr | 7 |
| pTSTE | apH(3′)I flanked by H. influenzae uptake sequences in pBR322; Ribr | 34 |
| pBJ100.1 | HindIII fragment of R2866 chromosome from position 139332 (in HI0123) to bp 5194 of the prophage in pUC18 | This study |
| pBJ100.2 | pBJ100.1 with TSTE located in BamHI site upstream of the prophage attP site | This study |
| pBJ102 | TrcHisB containing a 1.7-kb fragment from the 5′ end of the HP2 prophage | This study |
| pBJ102.2 | pPBJ102 containing a 2.0-kb fragment of the 3′ end of the HP2 prophage 3′ to the early fragment | This study |
| pBJ102.3 | apH(3′)I inserted between the two prophage fragments in pBJ102.2 | This study |
| pBJ102.4 | dCAT inserted between the two prophage fragments in pBJ102.1 | This study |
| pBJ105 | pKS containing the HindIII-EcoRI fragment of the HP2 prophage located in the pR transcription frame | This study |
| pBJ105.2 | pBJ105 with TSTE located in the NcoI site in dam | This study |