TABLE 2.
Modified nucleoside levels for parent strain MC1061, iscS mutant CL100(DE3), and CL100(DE3) complemented with plasmid pCL010a
| Strain | Relative level
|
|||||
|---|---|---|---|---|---|---|
| s2C | mnm5U | mnm5s2U | s4U | i6A | ms2i6A | |
| MC1061 | 1.0 (0.2) | (0.015) | 1.0 (0.11) | 1.0 (1.2) | NDb | (0.52) |
| CL100(DE3) | 0.02 | (0.15) | ND | ND | (0.65) | ND |
| CL100(DE3)/pCL010 | 0.9 | (0.01) | 0.7 | 1.0 | ND | (0.47) |
a
Levels of 2-thiocytidine (s2C), 5-methylaminomethyl-2-thiouridine (mnm5s2U), and 4-thiouridine (s4U) were measured as the area ratio to pseudouridine (Ψ) and then normalized relative to the parent strain set at 1.0. Parenthetical values were not normalized. Levels of 6-N-dimethylalyl-2-methylthioadenosine (ms2i6A) and i6A are reported relative to t6A and were not normalized to the parent strain. All nucleosides were quantified at 260 nm except 4-thiouridine, which was quantitated at 330 mm.
b
ND, none detected.