TABLE 3.
Levels of modified nucleosides in iscS mutant strains relative to the parent strain as a function of growtha
| Strain | Relative level
|
||||
|---|---|---|---|---|---|
| s2C | mnm5s2U | s4U | i6A | ms2i6A | |
| CL100, A600 = 0.5 | 0.01 | NDb | ND | 0.6 | ND |
| CL100, A600 = 1.0 | 0.4 | ND | ND | 0.5 | ND |
| CL100, t = 36 h | 0.7 | ND | ND | 0.2 | 0.2 |
| PK4331, A600 = 0.5 | 0.01 | ND | ND | 0.5 | ND |
| PK4331, t = 43 h | 0.9 | ND | ND | 0.1 | 0.1 |
| PK5930, A600 = 0.5 | 0.01 | ND | ND | 0.5 | ND |
| PK5930, t = 43 h | 0.7 | ND | ND | 0.1 | 0.1 |
a
2-Thiocytidine is reported as the area ratio to Ψ and normalized to the levels in MC1061, which was invariant with growth. Levels of 6-N-dimethylallyl adenosine (i6A) and 6-N-dimethylallyl-2-methylthioadenosine (ms2i6A) are reported directly as the area ratio to 6-N-threonylcarbamoyl adenosine (t6A). For other abbreviations, see Table 2, footnote a.
b
ND, none detected.