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. 2002 Dec;184(24):6751–6759. doi: 10.1128/JB.184.24.6751-6759.2002

TABLE 2.

Expression of lacZ transcriptional fusions from reporter plasmids in S. flexneri strains

Transcriptional fusion Reporter plasmida Size of the cloned fragment (bp) β-Galactosidase activity in derivatives of the following strainb:
Ratioc
Wild type ipaD
ipaH7.8-lacZ pMM11 584 30 1,700 55
ipaH4.5-lacZ pMM12 625 150 420 3
ipaH1.4-lacZ pMM13 568 45 40 1
ospC1-lacZ pMM19 740 30 440 15
ospF-lacZ pMM65 128 45 370 8
ospD3-lacZ pMM29 510 130 220 1.7
ospG-lacZ pMM20 338 220 250 1
icsB-lacZ pMM30 414 1,300 760 0.6
a

The reporter plasmids were constructed with vector pQF50 (Table 1).

b

β-Galactosidase activities assayed in derivatives of M90T (wild type) and SF622 (ipaD) are expressed in Miller units and are the means of at least four independent experiments. Standard deviations (not shown) were within 25% of the reported values.

c

Activity present in the ipaD mutant versus activity present in the wild-type strain.

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