. 2002 Dec;184(24):6751–6759. doi: 10.1128/JB.184.24.6751-6759.2002

TABLE 3.

Deletion analyses of ipaH9.8. ospC1, and virA promoters

Transcriptional fusion	Reporter plasmid^a	Coordinate of 5′ end of the cloned fragment^b	β-Galactosidase activity in derivatives of the following strain^c:		Ratio^d
Transcriptional fusion	Reporter plasmid^a	Coordinate of 5′ end of the cloned fragment^b	Wild type	ipaD	Ratio^d
ipaH9.8-lacZ	pMM10	−312	400	4,000	10
	pMM33	−63	480	1,400	3
	pMM34	−43	260	270	1
ospC1-lacZ	pMM19	−398	40	570	15
	pMM35	−63	110	1,100	10
	pMM37	−43	70	80	1
virA-lacZ	pBD7	−163	70	530	8
	pBD9	−43	135	55	0.4

The reporter plasmids were constructed with vector pQF50 (Table 1).

Coordinates are indicated with respect to the transcription start site.

β-Galactosidase activities assayed in derivatives of M90T (wild type) and SF622 (ipaD) are expressed in Miller units and are the means of at least four independent experiments. Standard deviations (not shown) were within 25% of the reported values.

Activity present in the ipaD mutant versus activity present in the wild-type strain.