TABLE 5.
Expression of lacZ transcriptional fusions from reporter plasmids in E. coli strains containing MxiE- and IpgC-producing plasmids
| Transcriptional fusion | Reporter plasmida | β-Galactosidase activity in derivatives of strains expressingb:
|
||
|---|---|---|---|---|
| MxiE | IpgC | MxiE + IpgC | ||
| ospC1-lacZ | pMM19 | 5 | 10 | 50 |
| ipaH7.8-lacZ | pMM11 | 190 | 280 | 5,500 |
| ospF-lacZ | pMM65 | 15 | 40 | 170 |
| ipaH4.5-lacZ | pMM12 | 50 | 90 | 45 |
| ospD3-lacZ | pMM29 | 20 | 60 | 50 |
a
The reporter plasmids were constructed with vector pQF50 (Table 1).
b
β-Galactosidase activities assayed in derivatives of E. coli strains harboring either pMM71 (MxiE), pKH128 (IpgC), or both (MxiE and IpgC) are expressed in Miller units and are the means of at least four independent experiments. Standard deviations (not shown) were within 25% of the reported values.