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. 2002 Dec;184(24):6751–6759. doi: 10.1128/JB.184.24.6751-6759.2002

TABLE 5.

Expression of lacZ transcriptional fusions from reporter plasmids in E. coli strains containing MxiE- and IpgC-producing plasmids

Transcriptional fusion Reporter plasmida β-Galactosidase activity in derivatives of strains expressingb:
MxiE IpgC MxiE + IpgC
ospC1-lacZ pMM19 5 10 50
ipaH7.8-lacZ pMM11 190 280 5,500
ospF-lacZ pMM65 15 40 170
ipaH4.5-lacZ pMM12 50 90 45
ospD3-lacZ pMM29 20 60 50
a

The reporter plasmids were constructed with vector pQF50 (Table 1).

b

β-Galactosidase activities assayed in derivatives of E. coli strains harboring either pMM71 (MxiE), pKH128 (IpgC), or both (MxiE and IpgC) are expressed in Miller units and are the means of at least four independent experiments. Standard deviations (not shown) were within 25% of the reported values.

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