TABLE 1.

Selected features of ICEF-encoded deduced gene products^a

ORF	No. of amino acid residues	Mass (Da)	pI	Cellular locationb	Feature(s)c
ORF1	239	28,164	10.86	C	Weak homology to short M. capricolum contig MC293
ORF2	63	7,235	10.59	C	Absent from ICEF-IA
ORF3	400	47,249	8.17	C	Contains Tral relaxase motif III (43)
ORF4	155	17,610	9.34	M
ORF5	739	84,981	10.04	M	TraG family (pfam02534; P = 3E-10); NTP-binding site
ORF6	62	7,549	10.36	C	Weak homology to putative exported protein (accession no. YP01989) of Y. pestis
ORF7	315	35,628	6.63	M
ORF8	79	9,481	10.42	C	Identical to ORF8a in first 52 residues
ORF8a	62	7,209	10.14	C	Present in ICEF-IA only
ORF9	288	34,455	9.77	C	Present in ICEF-IA only
ORF10	54	6,541	10.54	C	Present in ICEF-IA only
ORF11	238	27,904	4.27	C
ORF12	183	21,063	6.58	C	Single-stranded DNA-binding protein family (pfam00436; P = 4E-14)
ORF13	84	10,160	10.34	M
ORF14	522	61,800	5.82	LP	P57 lipoprotein; signal sequence
ORF15	93	9,987	9.22	M
ORF16	396	43,672	10.51	M	Frameshift detected in one type II element
ORF17	937	108,137	8.05	M	Homology to TraE/TrsE conjugation proteins; NTP-binding site
ORF18	227	26,633	6.94	M
ORF19	1,409	162,788	8.66	M	Frameshift in ICEF-IA; signal sequence
ORF20	159	19,023	9.59	C	Present in ICEF-IA only
ORF21	308	36,026	9.73	C
ORF22	388	45,786	10.24	C	Homology to M. pulmonis P16 (31% identity over 137 amino acid residues)

^aThe relative position and orientation of each orf are shown in Fig. 2.

^bAs predicted by the PSORT program (C, cytoplasm; M, contains transmembrane domain; LP, putative lipoprotein).

^cP represents the BLAST probability score for homology to consensus for protein families (pfam).