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. 2026 Jun 22;13(2):420–426. doi: 10.5455/javar.2026.m1044

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This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License (http://creativecommons.org/licenses/by/4.0)

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Figure 3. — Phylogenetic relationship of Jordanian Leptospira isolates (OK394051 and OK394045) based on the partial 16S rRNA (rrs) gene sequence. The evolutionary history was inferred using the Neighbor-Joining method. The bootstrap consensus tree inferred from 1000 replicates is taken to represent the evolutionary history of the taxa analyzed. Branches corresponding to partitions reproduced in less than 70% bootstrap replicates are collapsed. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) is shown next to the branches. The evolutionary distances were computed using the maximum composite likelihood method and are in the units of the number of base substitutions per site. Evolutionary analyses were conducted in MEGA X [4]. The Jordanian strains are nested within the pathogenic clade, showing close evolutionary proximity to L. interrogans, L. kirschneri, and L. borgpetersenii.