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. 2002 Dec;184(24):6768–6776. doi: 10.1128/JB.184.24.6768-6776.2002

FIG. 4.

FIG. 4.

Detection of the AdiS protein in different S. suis strains by immunoblot analysis. Whole-cell lysates of S. suis strains I9841/1 (lane 2), 10 (lane 3), 10ΔEF (lane 4), Sc. suis II (lane 5), D282 (lane 6), I4627 D (lane 7), and I4627 F (lane 8) and S. pyogenes strain Kiel 4875 (lane 1; positive control) were prepared from temperature-induced cultures as described in Materials and Methods and separated by SDS-PAGE. Proteins were transferred to membranes by Western blotting and then probed with a specific antiserum against the SAGP protein from S. pyogenes. In all strains a specific reaction was seen with a single band at approximately 53 kDa. Lane M, molecular size markers.