FIG. 5.

Detection of the AdiS protein in the membrane fraction of S. suis by immunoblot analysis. S. suis strain I9481/1 was grown as described in Fig. 1 under noninducing conditions (37°C) until early logarithmic phase, and then one-half of the culture was shifted to a temperature stress at 42°C. Noninduced and temperature-induced cultures were fractionated and separated by SDS-PAGE as described for Fig. 1. In addition, CMPs were included in the analysis. After separation by SDS-PAGE proteins were transferred to membranes by Western blotting and then probed with a specific antiserum against the SAGP protein from S. pyogenes. A specific reaction was seen with a single band at approximately 53 kDa, which was significantly stronger in the MAP and CMP fractions of the 42°C culture than in those of the 37°C culture. This difference was almost not seen in the PP fraction and was only moderate in the CSP fraction. Molecular sizes are given on the left.