TABLE 1.
Strains, plasmids, and phage used in this study
| Strain, phage, or plasmid | Relevant genotype or characteristics | Source or reference |
|---|---|---|
| AK3 | rpoS::tet in W3110 | Laboratory strain |
| BLS1 | ΔgabC (ygaE)::cat Δnac-28 | P1 lysate from SR5 (Kanr) into SR1 |
| BLS2 | ΔgabC (ygaE)::cat rpoS::tet | P1 lysate from SR1 (Camr) into AK3 |
| CP6 | ΔgabT | This work |
| EB3364 | Δnac-28::kan | 25 |
| HK1 | gabT::(mini-Tn5 lacZ-tet/1) in W3110 | P1 lysate from MT114 (Tetr) into W3110 |
| HK9 | Δnac::cat | This work |
| HK10 | Δnac::cat trp::(kan-gabD′-′lacZ) | P1 lysate from HK9 (Camr) into SR6 |
| K4633 | recD1903::Tn10 | D. Friedman (University of Michigan) |
| MT114 | gabT::(mini-Tn5 lacZ-tet/1) | 1 |
| SR1 | ΔgabC (ygaE)::cat in W3110 | This work |
| SR2 | ΔygaF::cat in W3110 | This work |
| SR4 | ΔgabDT::cat in W3110 | This work |
| SR5 | nac-28::Tn5 in W3110 | P1 lysate from EB3364 (Kanr) into W3110 |
| SR6 | trp::(kan-gabD′-′lacZ) in W3110 | This work |
| SR7 | trp::(kan-gabP′-′lacZ) in W3110 | This work |
| SR8 | trp::(kan-gabD′-′lacZ) ΔgabC (ygaE)::cat in W3110 | P1 lysate from SR1 (Camr) into SR6 |
| TE2680 | Δ(lac)X74 recD1903::Tn10 trpDC700::putPA1303::(cat-′lac) | 9 |
| W3110 | lacL8 lacIq | Laboratory strain |
| Kohara 443 | ygaF-gabDTP-ygaE (gabC) | F. Blattner (University of Wisconsin) |
| pACYC184 | cat | 3 |
| pGabp | Transcription template for gabD promoter | This work |
| pRS551 | kan-′lacZ | 42 |
| pWM529 | Cloning vector | 22 |