TABLE 6.
Transaminase and dehydrogenase activities and rates of carbon-limited growth
| Strain and genotype | Carbon sourcea | Activity (nmol min−1 mg of protein−1) ± SE (no. of determinations)
|
Avg doubling time (min) ± SEb | |
|---|---|---|---|---|
| Transaminase | Dehydrogenase | |||
| SR6 (wild type) | Glucose | 25.7 ± 1.4 (3) | 28.5 ± 3.5 (3) | 84 ± 11 |
| SR8 (gabC) | Glucose | 34.1 ± 6.1 (3) | 29.2 ± 5.1 (3) | 78 ± 6.4 |
| W3110 (wild type) | Glycerol | 38.8 ± 11 (3) | 32.7 ± 4.3 (3) | 104 ± 2.7 |
| SR1 (gabC) | Glycerol | 110 ± 17 (3) | 80.6 ± 9.4 (3) | 100 ± 3.7 |
| BLS1 (gabC nac) | Glycerol | 121 ± 6.9 (3) | 71.9 ± 5.2 (3) | 116 ± 9.1 |
| BLS2 (gabC rpoS) | Glycerol | 13.9 ± 0.47 (3) | 10.5 ± 0.29 (3) | 130 ± 7.8 |
| W3110 (wild type) | GABA | NGc | NG | NG |
| SR1 (gabC) | GABA | 738 ± 50 (3) | 434 ± 69 (3) | 236 ± 2.7 |
| BLS1 (gabC nac) | GABA | 1,500 ± 51 (3) | 779 ± 47 (3) | 245 ± 14 |
| BLS2 (gabC rpoS) | GABA | NG | NG | NG |
| W3110 (wild type) | Aspartate | 47.2 ± 7.2 (3) | 36.3 ± 4.9 (4) | 256 ± 3.3 |
| SR1 (gabC) | Aspartate | 1,000 ± 30 (3) | 534 ± 43 (2) | 310 ± 14 |
| BLS1 (gabC nac) | Aspartate | 1,700 ± 67 (3) | 961 ± 61 (3) | 344 ± 3.2 |
| BLS2 (gabC rpoS) | Aspartate | 63.2 ± 6.7 (3) | 33.1 ± 1.4 (3) | 336 ± 13 |
a
Cells were grown with the indicated carbon source at 0.4% and 0.2% (NH4)2SO4.
b
Doubling times of at least three cultures are shown.
c
NG, no growth.