FIG. 1.

RT-PCR analysis of gadX and gadW transcripts. Inset, left, represents the gadX and -W genes and the location of primers used for RT-PCR. gadX forward, oligo-340; gadX reverse, oligo-341; gadW forward, oligo-414; gadW reverse, oligo-415; intergenic forward, oligo-458; and intergenic reverse, oligo-459. PCRs with and without RT (Reverse Tntp′ase) were run, and products were separated on 2% agarose gels. Lane 1, HindIII-cut lambda DNA; lane 2, 100-bp ladder; conditions for lanes 3 to 12 are shown above the gel. RNA used was from log-phase (pH 5.5), LB-grown EK227 cells. Similar results were obtained using RNA from cells grown at pH 8 and minimal-glucose-grown cells (data not shown).