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Northern blot analysis of GadW effects on gadX message. Cells were grown to log phase in buffered LB medium. RNA was extracted; 5 μg was electrophoresed in formaldahyde-agarose gels and probed with an 827-bp radiolabled gadX probe that can detect gadAX and gadX transcripts. Strains and relevant genotypes are indicated in the figure. pH is given immediately over the blot.
