FIG. 3.

Distribution of ArfA and BgaA in the noncellulosomal fraction of C. cellulovorans. The SDS-PAGE gel was stained with Coomassie brilliant blue (A) or examined for α-l-arabinofuranosidase activity using MUAf (B) or for α-l-arabinopyranosidase activity using MUAp (C) by detection of fluorescence under UV illumination. Lane M, protein mass standard; lane 1, cellulosomal fraction from C. cellulovorans xylan-grown culture (10 or 20 μg) (15); lane 2, noncellulosomal fraction from C. cellulovorans xylan-grown culture (10 or 20 μg) (15); lane 3, purified rArfA from E. coli BL21 harboring pEARF29 (3 or 6 μg); lane 4, purified rBgaA from E. coli BL21 harboring pEBGA29 (3 or 6 μg).