TABLE 1.
Substrate specificities of rArfA and rBgaA
| Substratea | Sp act (U/mg of protein)b
|
|
|---|---|---|
| rArfA | rBgaA | |
| pNP-α-l-arabinofuranoside | 19.05 | ND |
| pNP-α-l-arabinopyranoside | ND | 5.32 |
| pNP-β-d-xylopyranoside | ND | ND |
| pNP-β-d-glucopyranoside | ND | ND |
| pNP-β-d-cellobiopyranoside | ND | ND |
| pNP-β-d-galactopyranoside | ND | 0.51 |
| oNP-β-d-galactopyranoside | ND | <0.001 |
| pNP-β-d-fucopyranoside | ND | 0.65 |
| pNP-β-lactopyranoside | ND | <0.001 |
| pNP-α-galactopyranoside | ND | ND |
| Arabinoxylan (oat-spelt xylan) | 0.59 | <0.01 |
| Xylan (birchwood) | 0.03 | <0.01 |
| Arabinan (sugar beet) | 1.89 | <0.002 |
| Arabinogalactan (larch wood) | <0.01 | 0.07 |
| Carboxymethylcellulose | <0.001 | <0.001 |
a
pNP, p-nitrophenyl; oNP, o-nitrophenyl.
b
Enzyme activity was measured after incubation in 50 mM phosphate buffer (pH 6.0) with each substrate at 37°C for 30 min. ND, not detected.