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Primer extension analysis of C. diphtheriae C7 (wild type) and C7 mntR mutant strain (C7R). Whole-cell RNA was isolated from strains grown to mid-log phase in HIBTW medium containing either 20 μM Mn2+ (+) or no added supplements (−). The arrow indicates the location of RNA transcripts. G, A, T, or C indicates the DNA sequencing ladder used for the identification of the transcriptional start site. Primer extension analysis was done as described in Materials and Methods.
