FIG. 4.

Gel mobility shift assays. A 202-bp ³²P-labeled DNA fragment (DNA insert in plasmid pPO2) was incubated at room temperature for 10 min in the presence or absence of MntR with the indicated supplements. The sample was then applied to a nondenaturing 5% acrylamide gel and electrophoresed in a 20 mM NaPO₄ buffer at 50 V for approximately 1 h. Gels were dried and analyzed by autoradiography. In panel A, 5X, 2X, and 10X indicate multiples of the concentration indicated to the left.