TABLE 1.
Growth in mPGT medium
| Strain | Avg OD600 in mPGT medium with indicated supplement(s)a
|
|||
|---|---|---|---|---|
| None | Mn | Fe | Fe + Mn | |
| C. diphtheriae C7 wt | 0.3 | 0.3 | 4.0 | 3.6 |
| C. diphtheriae C7 mntR | 0.2 | 0.4 | 4.2 | 4.0 |
| C. diphtheriae C7 mntA | 0.2 | 0.2 | 3.4 | 4.0 |
| B. subtilis CU1065 wt | 0.2 | 1.4 | 0.8 | 3.6 |
a
Ferrous sulfate (Fe) was added to mPGT medium at 5 μM, and MnCl2 (Mn) was added at 0.1 μM. Measurements were taken on 18- to 24-h cultures. Values are averages of three independent experiments, and no experiment varied by more than 15% from the average. OD600, optical density at 600 nm; wt, wild type.