Fig. 3.

Interaction Between ENO1 and PKM promotes PCNA upregulation. (A) Co-immunoprecipitation experiment indicated the binding between ENO1 and PKM. (B) Immunofluorescence staining showed the co-localization of ENO1 and PKM in the cytoplasm. (C) GST-pull down assay verified the binding between ENO1 and PKM. (D-E) Data from GEPIA database suggested positive correlations between ENO1 and PCNA, between ENO1 and Ki67 (D), between PKM and PCNA and between PKM and Ki67 (E) in HCC tissues. (F-G) Transfection efficiency of pcDNA3.1-ENO1 vectors (F) and PKM siRNA (G) in HepG2.2.15 cells was examined by Western blot. *** P < 0.001 vs. the Control group. ### P < 0.001 vs. the NC or the siNC group. (H-I) By Western blot and qRT-PCR, PKM silencing reduced PCNA and Ki67 mRNA and protein expression in HepG2.2.15 cells, but this effect was reversed by ENO1 overexpression. *** P < 0.001 vs. the Control group. ## P < 0.01 and ### P < 0.001 vs. the ENO1 group. ^^ P < 0.01 and ^^^ P < 0.001 vs. the siPKM group