Fig. 7.

Verification of ENO1 and PKM influences on HBV replication in HCC by regulating glycolysis in a mouse tumor model. (A-B) Western blot showed the effectively increased expression of ENO1 (A) and PKM (B) proteins in HepG2.2.15 cells by transfecting with lentiviral ENO1 overexpression vectors and lentiviral PKM overexpression vectors. (C) Images of the xenograft tumors in nude mice and the stripped tumor tissues. (D-E) ENO1 and PKM overexpression increased the volume (D) and weight (E) of the xenograft tumors, which was abrogated by 2-DG treatment. (F-G) By qRT-PCR and ELISA, 2-DG treatment reversed the promotion of ENO1 and PKM on HBV DNA replication (F) and HBsAg secretion (G) in xenograft tumors. *** P < 0.001 vs. the NC group. ### P < 0.001 vs. the ENO1 group. ^^^ P < 0.001 vs. the PKM group