TABLE 2.
Quantitation of nidogen-1, nidogen-2, and laminin γ1 chain in tissue extracts by radioimmunoassaya
| Genotypea | Amt of protein (pmol/g[wet weight]) in:
|
|||||
|---|---|---|---|---|---|---|
| Kidney
|
Skeletal muscle
|
|||||
| Nidogen-1 | Nidogen-2 | Laminin γ1 | Nidogen-1 | Nidogen-2 | Laminin γ1 | |
| +/+ | 777 ± 160 | 36 ± 6.7 | 50.2 ± 12.4 | 732 ± 72 | 16 ± 6.4 | 95 ± 30 |
| +/− | 703 ± 192 | 22.2 ± 4.4 | 48.2 ± 17.4 | 735 ± 137 | 10.5 ± 3 | 98 ± 19 |
| −/− | 639 ± 46 | <0.3 | 44.6 ± 8.8 | 679 ± 82 | <0.1 | 90 ± 8 |
a
+/+, +/−, and −/− refer to nidogen-2 genotypes, and amounts of proteins are shown as means ± standard deviations based on independent measurements of single extracts of each tissue and genotype (n = 5).