Figure 5.

Enzymatic Activity of Heterologously Expressed AOP2.

HPLC results (monitored at 229 nm) of purified desulfoglucosinolates from bacterial extracts containing heterologously expressed AOP2 fusion proteins are shown. All compound identities were confirmed by comparison of both retention times and UV light absorption profiles with those of authentic standards. 3-MSO, 3-methylsulfinylalkyl glucosinolate; 4-MSO, 4-methylsulfinylalkyl glucosinolate.

(A) Extract from 3-methylsulfinylalkyl glucosinolate treated with uninduced AOP2 bacterial extract.

(B) Extract from 3-methylsulfinylalkyl glucosinolate treated with AOP2 enzyme.

(C) Extract from 4-methylsulfinylalkyl glucosinolate treated with uninduced AOP2 bacterial extract.

(D) Extract from 4-methylsulfinylalkyl glucosinolate treated with AOP2 enzyme.