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. 2001 Mar;13(3):667–680.

Table 1.

Levels of Expression of α-Amylase and GAMyb in the Wild Type and the slender Mutant Barleya


Expression of α-Amylase–GUS
Expression of GAMyb–GUS
Hormone Treatment Wild Type slender Mutant Wild Type slender Mutant
No hormone 0.2 ± 0.0 8.7 ± 1.2 14.4 ± 2.0 20.5 ± 2.1
+GA3 16.6 ± 2.7 10.3 ± 1.2 22.9 ± 1.3 22.2 ± 3.8
a

Either the α-amylase or the GAMyb reporter construct was bombarded into slender mutant embryoless half-seed along with the internal control construct UBI–luciferase, using the same amount of effector and reporter constructs (1.14 μg per shot). Transformed half-seeds were incubated for 12 hr with or without 1 μM GA3. GUS activities (×10−3) ±se are presented.