Table 1.
Levels of Expression of α-Amylase and GAMyb in the Wild Type and the slender Mutant Barleya
|
|
Expression of α-Amylase–GUS
|
Expression of GAMyb–GUS
|
||
|---|---|---|---|---|
| Hormone Treatment | Wild Type | slender Mutant | Wild Type | slender Mutant |
| No hormone | 0.2 ± 0.0 | 8.7 ± 1.2 | 14.4 ± 2.0 | 20.5 ± 2.1 |
| +GA3 | 16.6 ± 2.7 | 10.3 ± 1.2 | 22.9 ± 1.3 | 22.2 ± 3.8 |
Either the α-amylase or the GAMyb reporter construct was bombarded into slender mutant embryoless half-seed along with the internal control construct UBI–luciferase, using the same amount of effector and reporter constructs (1.14 μg per shot). Transformed half-seeds were incubated for 12 hr with or without 1 μM GA3. GUS activities (×10−3) ±se are presented.