Figure 3.

Evolutionary Comparison between Members of MuDR/Mu Family.

(A) Unrooted relatedness tree of the TIRs of all sequenced Mu transposable elements. The left (L) and right (R) TIRs are shown; MuDR and related elements described in this study are shown in lightface, and the nonautonomous elements Mu1 to Mu8 are shown in boldface. The tree was generated by the UPGMA method using the Jukes-Cantor model for estimation of distances as implemented in the PAUP4.0b program (Sinauer, Sunderland, MA). Sequences were aligned manually with the aid of Sequencher 3.1.1 (GeneCodes, Ann Arbor, MI) software.

(B) Alignment of the 32-bp MURA transposase binding site. Elements were classified based on known genetic properties (Walbot and Rudenko, 2001). At the top (labeled W.T. for wild type) are the sequences for MuDR and MuDRzc. Next are the mobile Mu elements, then the hMuDR elements, and the nonmobile Mu elements. Dots represent matching bases, and dashes represent missing bases.