Table 2.

Quantification of hmudrB and mudrB Transcript Levels in Mutator and Non-Mutator Maize Lines

			Relative Expression in Probe Regions Indicated (%)b,c
Line	Mutator	MuDR Copiesa	4298–4496	4332–4496	4087–4222	3782–4222
hmudrB
a1-mum2d	No	0	1.0 (100)	1.0 (100)	1.0 (100)
bz2	No	0	2.9 (100)	3.9 (100)	2.9 (100)
bz2-mu1	No	>20e	3.3 (100)	4.0 (100)	2.8 (100)
bz2-mu2	Yes	9–11	24.3 (26.6)	NDf	ND
bz2-mu2	Yes	18–24	52.8 (28.8)	59.4 (29.0)	54.8 (31.7)
a1-mum2	Yes	1	3.7 (20.9)	3.7 (20.9)	4.4 (24.7)
a1-mum2	Yes	1(d201)	ND	1.2 (100)	1.4 (100)
a1-mum2	Yes	6–8	16.7 (25.8)	18.1 (25.9)	21.5 (27.5)
a1-mum2	Yes	10–12	26.7 (26.5)	ND	ND
mudrB
a1-mum2	No	0	0	0	0	0
bz2	No	0	0	0	0	0
bz2-mu1	No	>20e	0	0	0	0
bz2-mu2	Yes	9–11	9.6	ND	ND	ND
bz2-mu2	Yes	18-24	18.6	21.1	19.5	22.3
a1-mum2d	Yes	1	1.0	1.0	1.0	1.0
a1-mum2	Yes	1(d201)	0	0	0	0
a1-mum2	Yes	6–8	6.9	7.5	8.3	7.4
a1-mum2	Yes	10–12	10.6	ND	ND	ND

^aCopy number was estimated from the intensity of hybridization to the 4.7-kb SstI band of MuDR by DNA blot hybridization.

^bProbe identity to various hMuDR elements is indicated in Table 1.

^cPercentage is calculated as the contribution of hmudrB transcripts in the total pool of hmudrB plus mudrB transcripts in the sample. Details are provided in Methods.

^dHybridization to total RNA from this line was set as 1.0, and all other measurements are relative to it.

^eCopy number estimate from Walbot and Stapleton (1998).

^fND, not determined.