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. 2001 Mar;13(3):571–584. doi: 10.1105/tpc.13.3.571

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Copyright © 2001, American Society of Plant Physiologists

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Figure 6. — Expression of HC-Pro Prevents Silencing in Response to the Mobile Silencing Signal from a Silenced Rootstock.

(A) Diagram of experiment showing the effect of grafting a plant expressing both GUS and HC-Pro (T19 × HC-Pro) onto a GUS-silenced rootstock (6b5 × NT): the scion continues to express GUS.

(B) RNA gel blot showing the accumulation of GUS mRNA in T19 × HC-Pro plants before and after being grafted onto GUS-silenced rootstocks (6b5 × NT). RNA was isolated from the T19 × HC-Pro plants just before grafting (lane 1, Pre-graft). Four weeks after grafting, RNA was isolated from the T19 × HC-Pro scions (lane 2, Graft) and from the plants used as the source of the scions (lane 3, Control). Ethidium bromide staining of 25S rRNA is shown as a loading control.

(C) GUS-histochemical staining of a leaf from the scion in the grafting experiment shown in (A).

(D) RNA gel blot showing the accumulation of GUS small RNAs in T19 × HC-Pro scions before and after being grafted onto GUS-silenced rootstocks (6b5 × NT). The pre-graft, graft, and control RNAs described in (B) were fractionated to enrich for small RNA, as described in Methods, and examined for 21- to 25-nucleotide (nt) RNAs derived from the GUS gene. Ethidium bromide (EtBr) staining of the predominant band of RNA found in samples enriched for small RNA is shown as a loading control.