Table 2.
Peripheral blood counts from control IgG- and CTLA4-Ig treated, 16-mo-old, mdx mice
| Parameter Assayed | Normal Range (95% Interval) | Control IgG (SE) | CTLA4-Ig (SE) | P Value |
|---|---|---|---|---|
| CBC parameters | ||||
| WBC, 103/μL | 4.45–13.9§ | 7.0 (0.96) | 7.98 (2.63) | 0.46 |
| RBC, 106/μL | 6.1–10.7*; 7.14–12.2§ | 9.04 (0.64) | 8.89 (1.11) | 0.80 |
| HGB, g/dL | 10.8–19.2§ | 12.26 (0.80) | 12.30 (1.24) | 0.95 |
| HCT, % | 33.5–47.8*; 37.3–62§ | 42.02 (2.56) | 42.08 (3.61) | 0.98 |
| MCV, fL | 43.4–47.8*; 42.7–56.0§ | 46.60 (1.52) | 46.60 (48.48) | >0.99 |
| NRBC (100 WBC) | 0 | 0 (0) | 0 (0) | >0.99 |
| MCH, pg | 14.8–17.6*; 11.7–16.3§ | 13.58 (0.41) | 13.88 (0.40) | 0.28 |
| MCHC, g/dL | 29.3–35.9*; 24.6–34.9§ | 29.18 (0.38) | 29.18 (0.56) | >0.99 |
| Absolute data | ||||
| Absolute neutrophils, /μL | 530–3009§ | 836.2 (411.3) | 1702 (841.9) | 0.07 |
| Absolute band cells, /μL | 0 | 86.8 (90.3) | 235.4 (120.2) | 0.06 |
| Absolute lymphocytes, /μL | 3240–11150§ | 5073 (675.9) | 4580 (1376) | 0.49 |
| Absolute monocytes, /μL | 150–940§ | 722.0 (194.0) | 874.8 (304.3) | 0.38 |
| Absolute basophils, /μL | 0 | 0 (0) | 0 (0) | >0.99 |
| Absolute eosinophils, /μL | 10–420§ | 282.0 (72.6) | 48.0 (60) | 0.001 |
| Absolute reticulocytes, 103/μL | 639.4 (14.8) | 740.6 (84.5) | 0.03 | |
| Absolute platelets, 103/μL | 841–2159§ | 1775 (1080) | 1739 (774.9) | 0.98 |
| Absolute promyelocytes, /μL | 0 | 0 (0) | 0 (0) | >0.99 |
| Absolute myelocytes, /μL | 0 | 0 (0) | 0 (0) | >0.99 |
| Absolute metamyelocytes, /μL | 0 | 0 (0) | 0 (0) | >0.99 |
| Absolute promyelocytes, /μL | 0 | 0 (0) | 0 (0) | >0.99 |
| Differential data | ||||
| Neutrophils, % | 8–20* | 11.60 (4.83) | 21.20 (8.14) | 0.05 |
| Band cells, % | 0–5 | 1.20 (1.10) | 3.20 (1.92) | 0.08 |
| Lymphocytes, % | 76–91* | 72.60 (4.04) | 58.60 (13.35) | 0.06 |
| Monocytes, % | 0–4* | 10.40 (2.61) | 15.40 (6.95) | 0.17 |
| Basophils, % | 0–130§ | 0(0) | 0 (0) | >0.99 |
| Eosinophils, % | 0–3§ | 4.20 (1.64) | 1.60 (2.51) | 0.09 |
| Myelocytes, % | 0 | 0 (0) | 0 (0) | >0.99 |
| Metamyelocytes, % | 0 | 0 (0) | 0 (0) | >0.99 |
| RBC morphology | ||||
| Reticulocytes, % | 3.8–4.2* | 7.10 (0.53) | 7.65 (0.24) | 0.10 |
| Heinz bodies | 0 | 0 (0) | 0 (0) | >0.99 |
| Poikilocytosis | None | Not observed | Not observed |
Data for normal range values were obtained from Santos et al. (46; indicated by *) or from Charles River labs (47; indicated by §). n = 5 for each group. HCT, hematocrit; HGB, hemoglobin; MCH, mean corpuscular hemoglobin; MCHC, mean corpuscular hemoglobin concentration; MCV, mean corpuscular volume; NRBC, nucleated red blood cell; RBC, red blood cell; WBC, white blood cell.