Table 1.

Phenotypic Characterization of Double Mutants between tt12 and Other Pigmentation Mutants

Single Mutant				Double Mutant
Locus	Seed Body	Chalaza	Vanillina	Loci	Seed Body	Chalaza	Vanillina
tt1-1	Golden yellow	Brown	−/+	tt1-1 tt12-1	Dull tt1-1	Pale gray	−/−
tt2-1	Golden yellow	None	−/−	tt2-1 tt12-1	tt12-2	None	−/−
tt3-1	Grayish yellow	Pale gray	−/−	tt3-1 tt12-1	Dull tt3-1	Gray	−/−
tt4-1	Pale yellow	None	−/−	tt4-1 tt12-1	tt4-1	None	−/−
tt5-1	Lemon yellow	Pale gray	−/−	tt5-1 tt12-1	tt5-1	None	−/−
tt6-1b	Very pale brown	Pale brown	−/−	tt6-1 tt12-1	tt6-1	Brown	−/−
tt7-1b	Very pale brown	Pale brown	−/−	tt7-1 tt12-1	Yellow	Gray	−/−
tt8-1	Lemon yellow	None	−/−	tt8-1 tt12-1	tt8-1	None	−/−
tt9-1	Grayish beige	Blackc	−/+	tt9-1 tt12-1	tt12-1	Dark brown	−/−
tt10-1d	Pale brown	Brownc	+/+	tt10-1 tt12-1e	tt12-1	Pale brown	−/−
tt11-2	Pale brown	Pale brown	−/−	tt11-2 tt12-1	tt11-1	Pale brown	−/−
tt13-1	Pale brown	Dark brown	−/+	tt13-1 tt12-1	tt12-1	Dark brown	−/−
tt14-1d	Pale brown	Dark brown	+/+	tt14-1 tt12-1e	tt12-1	Dark brown	−/−
tt15-1	Grayish pale brown	Blackc	−/+	tt15-1 tt12-1	Pale brown	Dark brown	−/−
ttg1-1	Lemon yellow	None	−/−	ttg1-1 tt12-1	Dull ttg1-1	None	−/−
ban-2	Grayish green	Blackc	−/+	ban-2 tt12-1c	Pale ban	Dark brown	−/−
Ws-1	Brown	Dark brown	+/+
tt12-1	Dull pale brown	Pale brown	−/−

^aProanthocyanidin detection with vanillin treatment: seed body (whole seed except chalazal area)/chalazal area; +, presence of red pigments; −, absence of red pigments.

^bMature tt7 seeds and to a lesser extent tt6 seeds are slightly spotted.

^cThe dark pigmentation of the chalazal area is more extended than with the other genotypes, and seeds appear spotted around this area for tt9, ban, tt15, and ban tt12.

^dThe mutants are pale brown at harvest and darken during after-ripening until they are nearly identical to wild-type Ws-1 seeds.

^eBoth tt10 tt12 and tt14 tt12 darken very little with time, like tt12 but unlike tt10 and tt14.