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. 2001 Apr;13(4):943–952. doi: 10.1105/tpc.13.4.943

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Copyright © 2001, American Society of Plant Physiologists

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Figure 2. — Voltage-Dependent Properties of AKT3, AKT3/(p)KST1, KST1, and KST1/(p)AKT3.

(A) Representative current traces in response to voltage pulses from +40 to −150 mV of AKT3 (left traces), KST1 (second right traces), and the chimeric channels AKT3/(p)KST1 (second left traces) as well as KST1/(p)AKT3 (right traces). Voltage pulses were applied in 10-mV decrements from a holding voltage of −10 mV for AKT3 and KST1/(p)AKT3 and −20 mV for KST1 and AKT3/(p)KST1, respectively. Oocytes with similar expression levels have been selected. AKT3 as well as KST1/(p)AKT currents were recorded at pH_ext. 7.4, whereas KST1 and the chimera AKT3/(p)KST1 were recorded at pH_ext. 5.6.

(B) The steady state currents I_ss were derived from the data shown in (A) and plotted against the membrane voltage. Note pronounced outward currents in AKT3 and KST1/(p)AKT3 but not in KST1 and AKT3/(p)KST1 in (A) and (B).

(C) The open probabilities p_o in 30 mM K⁺ and pH 7.4 for KST1 (⋄), AKT3 (○), KST1/(p)AKT3 (•), and AKT3/(p)KST1 (♦) were plotted against the membrane voltage. Solid lines represent best Boltzman fits to the data. Error bars indicate standard deviation ().

Inline graphic — Voltage-Dependent Properties of AKT3, AKT3/(p)KST1, KST1, and KST1/(p)AKT3.

(A) Representative current traces in response to voltage pulses from +40 to −150 mV of AKT3 (left traces), KST1 (second right traces), and the chimeric channels AKT3/(p)KST1 (second left traces) as well as KST1/(p)AKT3 (right traces). Voltage pulses were applied in 10-mV decrements from a holding voltage of −10 mV for AKT3 and KST1/(p)AKT3 and −20 mV for KST1 and AKT3/(p)KST1, respectively. Oocytes with similar expression levels have been selected. AKT3 as well as KST1/(p)AKT currents were recorded at pH_ext. 7.4, whereas KST1 and the chimera AKT3/(p)KST1 were recorded at pH_ext. 5.6.

(B) The steady state currents I_ss were derived from the data shown in (A) and plotted against the membrane voltage. Note pronounced outward currents in AKT3 and KST1/(p)AKT3 but not in KST1 and AKT3/(p)KST1 in (A) and (B).

(C) The open probabilities p_o in 30 mM K⁺ and pH 7.4 for KST1 (⋄), AKT3 (○), KST1/(p)AKT3 (•), and AKT3/(p)KST1 (♦) were plotted against the membrane voltage. Solid lines represent best Boltzman fits to the data. Error bars indicate standard deviation ().