Figure 7.

RNA Gel Blot Analysis of Selected Transcripts.

(Top) Total RNA for control and stressed roots (15 min to 7 days) was used for RNA gel blot analysis (10 μg/lane) for four transcripts: Osr40c1 (transcript 1129 [A]), subtilisin-chymotrypsin inhibitor 2 (transcript 252 [B]), sucrose synthase 2 (transcript 1249 [C]), and glycine-serine–rich protein-1 (transcript 1723 [D]). Signal intensities were analyzed by GelExpert software version 3.5 (Nucleotech).

(Bottom) Expression ratios [M, microarray (stressed/nonstressed)/N, RNA gel blot analysis (Cy5, stressed/Cy3, nonstressed] for each clone [A] to [D] for 0 min, 15 min, 1 hr, 3 hr, 6 hr, 24 hr, and 7 days) are listed.