Figure 1.

Constructs Used in This Study.

Sec-GFP, the secreted form of GFP in which the Arabidopsis chitinase B signal peptide (s.p.) was fused to GFP5 (GFP); CPY-GFP, the signal peptide and the N-terminal propeptide from the yeast CPY (detailed) were fused to GFP5; GFP-Chi, the Ct-VSD of the tobacco chitinase A (detailed) was fused to the secreted form of GFP (Sec-GFP); aleu-GFP, the CPY signal peptide was fused with petunia aleurain VSD (detailed) to the N terminus of GFP5. For comparison (in parentheses), the corresponding sequence of the previously described VSD from barley proaleurain (Holwerda et al., 1992) is shown. Underlined amino acids are essential for vacuolar targeting.