Table 1.

Oligonucleotide Primers

Name	Gene(s)	Location	Length (nucleotide) Degeneracy	Sequencea
PRF-1S	Profilin family	Codons 1–11	33/2 × 106	ATG(A/T)(G/C)NTGGCARNNNTAYGTNGAYGANCAYYTNATGT
PRF1-5′S4	PRF1	−476 bp from ATG	24 mer	GAAAGTATTATGATAAAGAAGGAT
PRF1-1A	PRF1	Codons 1–6	38 mer	ACCCGGGGATCCGTCGACACGTATGATTGCCAAGACAT
PRF-71A	Profilin family	Codons 71–78	24/2 × 103	YTCNCCYTGNAYNACCATRTAYTT
PRF-104A	Profilin family	Codons 104–113	32/2 × 105	TTRCAYTGNCCNC/GC/GNGTNAYNGGYTCNTCRTA
PRF1-180S	PRF1	−177 to −148 bp from ATG	29 mer	GCTTATTTTAAGGGCTAATTAATTGA
PRF1-143S	PRF1	−119 to −142 bp from ATG	26 mer	GCAGTTGACGAAAGTTTTTTTTATTA
LB102A	T-DNA	102 bp from end of left TDNA border	29 mer	GATGCAATCGATATCAGCCAATTTTAGAC
LB194A	T-DNA	194 bp from end of left TDNA border	30 mer	AACTGTAATGACTCCGCGCAATATTTACAC

^aR = purine, Y1 = pyrimidine, N = all four bases. Most primers can be found on prf1-1 map in Figure 1.