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. 2001 May;13(5):1063–1078. doi: 10.1105/tpc.13.5.1063

Figure 2.

Figure 2.

RF2 Protein Accumulates in Mitochondria.

(A) Homogenate (Hg), purified chloroplastic (Ch), microsomal (Ms), cytosolic (Cy), and mitochondrial (Mt) fractions of Ky21 seedlings were subjected to SDS-PAGE and stained with Coomassie blue.

(B) A duplicate gel was transferred to a nitrocellulose membrane and reacted with affinity-purified anti-RF2 antibodies.

(C) Protein extracts from isolated mitochondria of the inbred line Ky21 were treated with Triton X-100 and papain as indicated, subjected to SDS-PAGE, transferred to a nitrocellulose membrane, and reacted with affinity-purified anti-RF2 antibodies.

(D) Purified mitochondria from Ky21 seedlings were subjected to sonication and centrifugation to isolate the soluble and membrane fractions and then subjected to immunoblot analysis. Mt, purified mitochondria; Inf, insoluble fraction; Sf, soluble fraction.