Figure 5.

RNA Gel Blot Analysis of AtHDA6 Expression in axe1 Mutants.

(A) Analysis of total RNA (20 μg) from 8-day-old seedlings of wild-type Col (wt), mutants (M), and internal controls (C) for each mutant line (axe1-3, axe1-1, axe1-2, axe1-5, and axe1-4). The blot was probed with both a radiolabeled PCR product from the AtHDA6 gene and a radiolabeled Actin-2 cDNA, which was used as a loading control.

(B) Analysis of polyadenylated RNA (400 ng) from seedlings of wild-type Col (wt) and axe1-4 mutants (M). The blot was probed with both the AtHDA6 PCR product and a cDNA from EF1α, which was used as a loading control.