Figure 6.

RNA Gel Blot Analysis of Auxin-Regulated Genes in axe1 Mutants.

Analysis of total RNA (20 μg) from 8-day-old seedlings that were treated with either water (−NAA) or water containing 50 μM NAA (+NAA) for 2 hr. Seedlings from wild-type Col (wt), mutants (M), and internal controls (C) for each mutant line (axe1-3, axe1-2, axe1-5, and axe1-4) were analyzed. The blots were probed with cDNAs from GUS, HPH, an Arabidopsis GH3 homolog (AtGH3-2), the SAUR-AC1 gene (SAUR), and eight Aux/IAA genes (IAA1, -2, -3, -4, -5, -6, -11, and -13). The Actin-2 gene was used as a loading control.