Figure 3.

Effects of ABA on the Germination and Growth of Transgenic Plants.

(A) to (C) Seeds from transgenic lines carrying the pTA211 vector (A), from transgenic lines carrying the AtPLC1 antisense gene (B), and from transgenic lines carrying the AtIP5PII sense gene (C) were incubated on plates containing 0 μM (open squares), 2.5 μM (closed squares), 5 μM (open circles), and 10 μM (closed circles) ABA under normal light conditions (see Methods) for 1 to 6 days in the presence of Dex (30 μM). Germination frequency was measured by scoring for radicle emergence. Average values ±sd are shown for three independent lines of each construct. Approximately 100 to 150 seeds of each line were plated in duplicates.

(D) Transgenic plants were grown in ABA (2 μM) for 18 days in the absence (−) or presence (+) of Dex (30 μM). Representative plants of three lines for each transgenic construct tested and a vector control transgenic line are shown. .