Figure 9.

Expression of Photosynthetic Genes Is Not Downregulated in sxd1 Mutant Leaves.

(A) Total RNA was extracted from the tip (T), middle (M), and base (B) of the third leaf of 18-day-old wild-type (WT) and sxd1 seedlings. Leaf sections from three different plants were combined before extraction of RNA. The same blot was hybridized sequentially with probes specific for LHCP, RBCS, and PEPCase, and the levels of rRNA served as a control for loading.

(B) Protein gel blots comparing levels of photosynthetic proteins in the tip of leaf 3 from 18-day-old wild-type and sxd1 seedlings. The lower panel shows a Coomassie-stained gel showing the protein profiles in the samples used for protein gel blot analysis. Each sample consisted of total protein extract pooled from leaves excised from three plants. At left, molecular weight markers.