Figure 1.

Harpin_Psph-Induced Hypersensitive Cell Death and PR Gene Expression in Tobacco.

Harpin_Psph (1 μM) was infiltrated into tobacco leaves or added to suspension cultured tobacco cells.

(A) Tobacco leaf treated with 5 mM Mes buffer, pH 5.5 (1), or harpin_Psph (2) 2 days after infiltration.

(B) Total RNA prepared from tobacco cells treated for 3 hr with buffer (−) or harpin_Psph (+) was used as a template in RT-PCR assays with DNA primers derived from the tobacco genes indicated (see text). The tobacco gene encoding EF1α served as an internal control. PZ, chitinase/lysozyme.

(C) Kinetics of HIN1 expression in tobacco cells treated with buffer or harpin_Psph. Total RNA from tobacco cells was prepared at the times after infiltration indicated and used in RT-PCR to simultaneously amplify HIN1 and EF1α transcripts.

(D) HIN1 expression in tobacco cells or leaves treated for 3 hr with buffer (−) or harpin_Psph (+). RT-PCR analysis was performed as described in (C).