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Creation of a Superactive SOS2 Kinase by Combining the Thr168-to-Asp Mutation with the Autoinhibitory Domain Deletion.
Construction of the SOS2 mutant proteins was as shown in Figure 9A.
(A) Coomassie blue–stained SDS-PAGE gel containing the relevant proteins.
(B) Kinase autophosphorylation.
(C) Phosphorylation of p3 by the kinases. Error bars indicate ±sd (n = 3).
