Figure 7.

Interaction between the Kinase and Regulatory Domains within the SOS2 Protein.

Wild-type SOS2 and its deletion constructs depicted in Figure 1 were transformed into yeast strain Y190 harboring pAS-SOS2N as bait. Shown are β-galactosidase assays on a filter (top) or in liquid culture (bottom) for quantification of relative interaction strength. Numbers at left represent arbitrarily defined unit U. Error bars indicate ±sd.